Exclusive Production of Gentamicin C1a from Micromonospora purpurea by Metabolic Engineering
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چکیده
منابع مشابه
Gentamicin formation in Micromonospora purpurea: stimulatory effect of ammonium.
The effect of ammonium on the fermentative production of gentamicin in Micromonospora purpurea has been studied using a chemically defined medium. Ammonium chloride concentrations ranging from 20 to 150 mM resulted in a proportional stimulation of growth and antibiotic formation. The use of other ammonium salts exerted a similar effect. Among the products of ammonium assimilation, glutamate and...
متن کاملThe gentamicin antibiotics. 6. Gentamicin C2b, an aminoglycoside antibiotic produced by Micromonospora purpurea mutant JI-33.
A mutant strain of Micromonospora purpurea, designated var. JI-33, produced an antibiotic complex consisting primarily of gentamicin C1a. A further product of this fermentation was identical to a very minor component isolated from the fermentation of the parent organism and named gentamicin C2b. Physical measurements indicated its structure to be 6'-N-methylgentamicin C1a and this was confirmed...
متن کاملOptimization of nutritional requirements for gentamicin production by Micromonospora echinospora.
Effect of various fermentation media, carbon sources, nitrogen sources, phosphate concentration and culture requirements includes inoculum levels and age were determined on gentamicin production and biomass dry weight production for Micromonospora echinospora, a gentamicin producing strain. Of the substrates tested, starch as a sole carbon source promoted maximal gentamicin production, while ma...
متن کاملCharacteristics of bacteriophages for Micromonospora purpurea.
Chemical and physical stabilities of bacteriophages øUW 21 and øUW 51 infecting Micromonospora purpurea ATCC 15835 were examined. Both phages were stable over the pH range of 5 to 8 and to heating at temperatures up to 50 degrees C and especially stable in buffer containing magnesium ion. Exposure to 1 M Ca(NO3)2 inactivated both phages, and phage øUW 51 was also susceptible to 1 M CaCl2, 0.1 M...
متن کاملBiosynthetic pathway leading to gentamicin C2b.
Incubation of Micromonospora purpurea SC 1210 (NRRL 5467) with L-[methyl-14C]methionine yielded [methyl-14C]gentamicin A and methyl-14C-labeled antibiotic JI-20A in a molar radioactivity ratio of 9:2. We did not isolate methyl-14C-labeled gentamicin X2, which was expected as an intermediate based on the biosynthetic pathways proposed by others. Addition of methyl-14C-labeled antibiotic JI-20A t...
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ژورنال
عنوان ژورنال: Antibiotics
سال: 2019
ISSN: 2079-6382
DOI: 10.3390/antibiotics8040267